FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n01_WGS17-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n01_WGS17-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557804
Sequences flagged as poor quality0
Sequence length28
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG454.0736631E-722.0000026
GTGTCAT454.0736631E-722.0000025
GTCCGTG454.0736631E-722.0000026
ATCGCTG454.0736631E-722.0000026
AGGTATG555.2386895E-922.06
GGGGATG605.966285E-1022.06
CGGAATG250.002578711822.06
AACCGTG250.002578711822.06
CGGGTCA250.002578711822.02
CAGAACA250.002578711822.03
CTATGAG250.002578711822.04
GGGGAAT250.002578711822.05
GTCGCTG353.2113523E-522.06
AGTCCTG302.870093E-422.06
AGTCCTC250.002578711822.03
CCTTAGT353.2113523E-522.05
TGCCCTT353.2113523E-522.03
CCCTCTG353.2113523E-522.06
TCCGCTG403.609768E-622.06
ACTGCCC353.2113523E-522.022
Produced by FastQC (version 0.11.5)