FastQCFastQC Report
Fri 28 Aug 2020
000000000-G69NY_l01_n01_WGS17-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NY_l01_n01_WGS17-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501118
Sequences flagged as poor quality0
Sequence length28
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGTT403.608553E-622.0000025
AGGTAGA302.8695283E-422.0000022
GCCTTTG302.8695283E-422.0000026
TCGCATG302.8695283E-422.0000026
GCAAGTG403.608553E-622.0000026
TCACCTG302.8695283E-422.0000026
CGAAATG403.608553E-622.0000026
CATGCTG302.8695283E-422.0000026
AGCCCTG302.8695283E-422.0000026
GGCACTG302.8695283E-422.0000026
GCGAAAT302.8695283E-422.0000025
CTTCACT302.8695283E-422.0000025
AGCCATG302.8695283E-422.0000026
TCGGGTG605.966285E-1022.0000026
TGAACTG403.608553E-622.0000026
GTGATTG403.608553E-622.0000026
TCTAGCT302.8695283E-422.0000025
ACCTCTG302.8695283E-422.0000026
AGAGTAT302.8695283E-422.0000025
ACCTATG403.608553E-622.0000026
Produced by FastQC (version 0.11.5)