FastQCFastQC Report
Fri 11 Sep 2020
000000000-G69NV_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NV_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949047
Sequences flagged as poor quality0
Sequence length287
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT75330.7937436185984467No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT60180.6341097964589741No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT58440.6157756149063218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGACA11700.0277.397436
TGATGAC11750.0275.021275
GCGATAC8650.0274.50292
TGACAGC11850.0273.886088
CTATCGA9250.0271.886474
GCTATCG9250.0271.886473
GATACGA8900.0271.52814
ATGACAG12050.0269.340247
CCGCTAT9650.0267.89641
TATCGAC9350.0267.475925
CGCTATC9500.0266.210542
GGCGATA9100.0265.560461
TACGACA9000.0263.82786
TCGACAG9850.0252.472087
CGAAAGC150.003295861187.333338
TGACATC700.0180.642878
GTCTCTC1050.0147.190481
ATATGAC608.094503E-10140.55
GAAAGCA504.479807E-8140.59
AATGATG200.0077916034140.53
Produced by FastQC (version 0.11.5)