FastQCFastQC Report
Fri 11 Sep 2020
000000000-G69NV_l01_n02_JRS_018_C9T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NV_l01_n02_JRS_018_C9T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307854
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6738721.889272187465487No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6713921.808714520519466No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT5125516.649125884347775No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG24920.8094746210866189No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC14600.4742507812144718No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG14410.4680790244726396No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG14080.45735965749998375No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG13360.43397194774146186No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC12790.4154566775159654No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC11960.38849584543322485No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAT11880.3858972110156113No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG10020.3254789608060964No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT9160.29754364081675083No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACATCCTATG9070.29462017709693555No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC9010.2926712012837254No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAATTCCACCA8110.26343656408557303No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGC7670.24914407478869854No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG7390.24004885432705111No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGTCATGGGCGAGCGC6830.2218584134037563No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG6760.2195846082883445No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG6730.2186101203817394No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGCG6680.21698597387073093No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGATGGGCGAGC6280.20399280178266319No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT6250.20301831387605812No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG5760.18710167806817518No Hit
GTACTGATGACAGCAGTACTACTGACGATGGGACAGCAGTGTGTGAGGCT5630.1828788971395532No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG5380.17475816458451082No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC5260.17086021295809053No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACATATGGGA4730.1536442599414008No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGAGCAGTGCGA4720.15331943063919912No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACAGCAGTATCATCGAGC4680.15202011343039235No Hit
GTACTGATGACAGCAGTATCTGCTGTCGATATCTGCTCGCCGATATATGG4630.15039596691938387No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACGATACTGC4610.14974630831498048No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGGCGAGCGCTATGAGCGAC4600.14942147901277877No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACCTATGGGCGAG4550.14779733250177032No Hit
CCGCTATCGACAGCAGTATCTGCTGTCAGTATTATCCGTCTATACGGATA4510.14649801529296352No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCATG4510.14649801529296352No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG4500.14617318599076187No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG4340.14097591715553476No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGATCATCAGCATGGATCGA4250.13805245343571954No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCAGT4230.13740279483131615No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGT4200.13642830692471106No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAACTATGGGC4180.1357786483203077No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA4170.135453819018106No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC4090.13285518460049245No Hit
GTACTGATGACAGGACAGCAGTGTGTGAGGCTATGGGAGAGCATAGACAT4000.12993172088067723No Hit
GTACTGATGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG3920.12733308646306365No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA3920.12733308646306365No Hit
GTACTGATGACAGCAGTATCTGCTGTGGGCAGTGGCGAGCGCTATGAGCG3900.12668342785866027No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG3900.12668342785866027No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCACTA3880.12603376925425688No Hit
GTACTGATGACAGTACTATGGGAGAGCGCGCTCGTCTATGGGTCAGCAGT3820.12408479344104674No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC3720.1208365004190298No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT3710.12051167111682812No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGATCGACAAATGGCAGTATT3690.11986201251242472No Hit
CTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGCTATGGGAGAGCG3630.11791303669921457No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC3630.11791303669921457No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAGTGTGTGAGTCT3580.1162888901882061No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGGGA3530.11466474367719764No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC3500.11369025577059255No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG3390.11011713344637392No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA3390.11011713344637392No Hit
CCGCTATCGACAGCAGTATCTGCTGTCTATGGGAGAGCGCGCTCGTCGAC3390.11011713344637392No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAGTATGGGAC3360.10914264553976885No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC3300.1071936697265587No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC3250.10556952321555023No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC3170.10297088879793667No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGAGAGCGCGCT3160.10264605949573499No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA3100.10069708368252482No Hit
GTACTGATGACAGCAGTATCTGCCGAGCCCATCTGGGCGAGCGCTATGAG3100.10069708368252482No Hit
GTACTGATGACAACAGCTCGTCGACTATGGGTCAGCAGTACTACTGACGA3090.10037225438032314No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGACA112400.0279.8756
TGACAGC111000.0279.607678
TGATGAC113200.0279.26245
CGATACG85950.0279.038423
GGCGATA85600.0278.70211
CCGCTAT84050.0278.659731
CTATCGA84000.0278.658334
ATGACAG112650.0278.630287
GCTATCG84150.0277.994633
GCGATAC86200.0277.740142
GATACGA86500.0277.426574
GTACTGA114450.0277.317171
ATACGAC86550.0277.266335
CTGATGA114150.0277.18444
ACTGATG114550.0276.707123
TATCGAC84700.0276.355355
TACTGAT115150.0275.14332
TACGACA87000.0275.024726
CGCTATC85500.0273.76962
TCGACAG84800.0272.881477
Produced by FastQC (version 0.11.5)