FastQCFastQC Report
Fri 11 Sep 2020
000000000-G69NV_l01_n02_JRS_018_C9A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NV_l01_n02_JRS_018_C9A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156561
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT4317027.57391687585031No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT3245320.728661671808432No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT2836918.120093765369408No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG6040.3857921193656147No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG4960.31680942252540545No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC4930.31489323650206624No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC4670.2982862909664603No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC4520.28870536084976467No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAGTGTGTGAGTCT4050.2586851131507847No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC4040.25804638447633826No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG4020.25676892712744553No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA3650.2331359661729294No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACTATGGGA3420.21844520666066264No Hit
CCGCTATCGACAGCAGTATCTGCATGGGTACAGCAGTATCTATGGGACAG3390.2165290206373235No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT3170.20247698979950307No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGAG3010.19225733100836095No Hit
GTACTGATGACAGCAGTATCTATGGGAGAGCGCGCTCGTCGACTATGGGT2970.18970241631057544No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA2900.18523131558945075No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG2860.18267640089166523No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG2760.17628911414720141No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCGAG2690.17181801342607675No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTACTAT2580.16479199800716654No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG2540.162237083309381No Hit
GTACTGATGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG2520.16095962596048827No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA2510.16032089728604185No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTATGCTATGGGAGAGC2490.1590434399371491No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACG2450.15648852523936357No Hit
GTACTGATGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG2440.15584979656491718No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT2430.15521106789047082No Hit
GGCGATACGACAGCAGTATCTGCTGTCATCTATGGGAGAGCGCGCTCGTC2420.15457233921602442No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA2340.14946250982045337No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC2310.14754632379711421No Hit
GTACTACTATGGGAGAGCGCGCTCGTCGACTATGGGTCAGCAGTACTACT2310.14754632379711421No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGACTATGGGCGACTATGGGAC2290.14626886644822146No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG2250.14371395175043594No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC2220.1417977657270968No Hit
GTACTGATGACAGCAGTATCCTATGGGAGAGCGCGCTCGTCGACTATGGG2210.1411590370526504No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAG2170.13860412235486488No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT2150.1373266650059721No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG2120.13541047898263298No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG2070.13221683561040104No Hit
CTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTATCATCGACTAT2050.1309393782615083No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG2050.1309393782615083No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA2040.13030064958706192No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG2010.12838446356372277No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG2000.1277457348892764No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA2000.1277457348892764No Hit
GTACTGATGCTATGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTATC1990.12710700621483No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACATGGGACTATG1980.12646827754038362No Hit
GGCGATACGACAGCAGTATCTACTATGGCTATGGGAGAGCGCGCTCGTCG1830.11688734742368789No Hit
GGCTATGGCTATGGCGACTATGACCTATGGGTCAGCAGTACTACTGACGA1810.11560989007479512No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG1800.11497116140034874No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG1770.1130549753770096No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT1760.11241624670256321No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG1760.11241624670256321No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT1720.1098613320047777No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCACG1720.1098613320047777No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACGATGGGA1700.10858387465588493No Hit
GTACTGATGACAGCAGTATCTGCTGTCTCCCATATGGGAGAGCTATGGGT1680.10730641730699216No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG1650.10539023128365302No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATTCTATGGGAGAGCATAGACAT1640.10475150260920664No Hit
GTACTGCTATGGGCGAGCGCTATGAGCGACTATGGGACCTATGGGAGAGC1630.10411277393476025No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA1610.1028353165858675No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT1600.1021965879114211No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGC158.776715E-6281.06
CCGCTAT41250.0280.31881
GATGACA56850.0280.011446
GCTATCG41300.0279.979433
ATGACAG57050.0279.768627
GGCGATA39400.0279.57361
TGACAGC57100.0279.27768
GATACGA39450.0279.219274
CGATACG39600.0278.871223
ACTGATG58000.0278.819823
TACTGAT58000.0278.819822
GTACTGA58200.0278.58591
GCGATAC39700.0277.814852
CTATCGA41700.0277.63074
TGATGAC57750.0277.593965
ATACGAC39750.0277.465425
CGCTATC41800.0276.630372
TCGACAG41900.0276.305487
TACGACA39950.0276.076326
TATCGAC41900.0275.970185
Produced by FastQC (version 0.11.5)