FastQCFastQC Report
Fri 11 Sep 2020
000000000-G69NV_l01_n02_JRS_018_C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NV_l01_n02_JRS_018_C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591700
Sequences flagged as poor quality0
Sequence length287
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT12876621.762041575122527No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT10491017.730268717255367No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT9238015.612641541321615No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAATTTTCTCTTAA28760.48605712354233566No Hit
GTCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT28350.4791279364542843No Hit
CCGCATGTCTGACCTTTTGCAGGTGCAGCCTTCCACTGCGAATCATTAAA19740.33361500760520535No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACG18230.30809531857360145No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAGTGTGTGAGTCT18000.3042082136217678No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG17350.2932229170187595No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC14280.2413385161399358No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG14080.2379584248774717No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC12530.211762717593375No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTACTAT12380.20922764914652697No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGAG12080.20415751225283083No Hit
CTGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT11730.19824235254351869No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG11320.1913131654554673No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA11010.18607402399864797No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTATGCTATGGGAGAGC10560.17846881865810377No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT10330.17458171370627007No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG10230.17289166807503803No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG9930.16782153118134188No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA9830.16613148555010984No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT9610.16241338516139936No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC9390.15869528477268888No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA9270.1566672300152104No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC8870.14990704749028222No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG8770.14821700185905018No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAA8070.1363866824404259No Hit
GTACTGAATGTGATCATTAGGAAGATTACGAAAAATATAAAAAATATGAG7600.1284434679736353No Hit
CCGCTATCGACAGCAGTATCTGCATGGGTACAGCAGTATCTATGGGACAG7590.12827446341051207No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCATGG7560.12776744972114248No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG7340.12404934933243199No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG7300.12337333107993916No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGAGTATG7260.12269731282744635No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACAGCACGACAGTCGACT7260.12269731282744635No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG7170.1211762717593375No Hit
GTACTGATGACAGCAGTATCTATGGGAGAGCGCGCTCGTCGACTATGGGT7080.11965523069122866No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC7080.11965523069122866No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA6810.11509210748690214No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACGATGGGA6760.11424708467128614No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG6590.11137400709819165No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCGAG6460.10917694777759No Hit
CCGCTATCGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG6440.10883893865134357No Hit
CCTCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6340.10714889302011153No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACC6320.10681088389386513No Hit
CCGCTATCGACAGCAGTAGTACGATTCTTTGATTATGTAATAAAATGTGA6280.10613486564137233No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACAGCACGACAGTCGACT6180.10444482001014029No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGCTATGGGCGA5980.10106472874767619No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGACGACT5930.10021970593206016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA149450.0280.717961
CCGCTAT166750.0280.073151
TGATGAC201100.0279.882175
GCGATAC149850.0279.59362
GATGACA200750.0279.530276
CGCTATC168200.0279.329352
TGACAGC202200.0279.262858
GTACTGA204250.0279.005131
TACTGAT203500.0278.514532
GCTATCG169700.0277.853883
ACTGATG203550.0277.686833
CTATCGA171550.0276.986884
TCGACAG170400.0276.382637
CTGATGA203850.0276.106484
CGACAGC329250.0275.964638
TATCGAC172950.0274.338535
CGATACG158650.0272.941073
ATGACAG207800.0272.21037
TACGACA160400.0272.153056
GATACGA160400.0271.71514
Produced by FastQC (version 0.11.5)