FastQCFastQC Report
Fri 11 Sep 2020
000000000-G69NV_l01_n02_JRS_018_C8G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NV_l01_n02_JRS_018_C8G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337119
Sequences flagged as poor quality0
Sequence length287
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT9376927.814807234240728No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6966120.663623230965918No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT6508019.304755887386946No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC23790.7056855294421258No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC14800.43901411667690043No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG12710.3770182042542841No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG11500.3411258339043483No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT11400.3381595223051801No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG11230.33311679258659405No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG11140.3304471121473426No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG9110.27023098668422724No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC9040.26815456856480946No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC8480.25154322360946724No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC8260.2450173380912971No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG8130.24116113301237843No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACTATGGGA7850.23285546053470732No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT7380.21891379601861657No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA7170.21268454166036327No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCTAT7140.21179464818061278No Hit
GTACTGATGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG6940.2058620249822763No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA6910.2049721315025258No Hit
CCGCTATCGACAGCAGTATCTGCATGGGTACAGCAGTATCTATGGGACAG6580.1951833032252706No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC6470.19192036046618552No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCGAG6200.18391131914843126No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG6130.1818349010290135No Hit
GTACTGATGACAGCAGTATCTGCTGTCACTATGGGCGAGCGCTATGAGCG6070.18005511406951255No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG5960.1767921713104275No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC5840.17323259739142557No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGAG5560.1649269249137545No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTACAGTCGCTACGACT5510.16344376911417036No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT5290.15691788359600023No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG5280.1566212524360834No Hit
GTACTGATGACAGCAGTATCTCTATGGGAGAGCGCGCTCGTCGACTATGG4960.147129055318745No Hit
CCGCTATCGACAGCAGTATCTGCTGTCCTATGGGCGAGCGCTATGAGCGA4900.14534926835924408No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA4730.14030653864065806No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC4660.13823012052124026No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG4640.13763685820140661No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCGCTAT4620.137043595881573No Hit
GTACTGCTATGGGCGAGCGCTATGAGCGACTATGGGACCTATGGGAGAGC4530.13437391544232155No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT4500.1334840219625711No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGCG4410.13081434152331967No Hit
CCGCTATCGACAGCAGTATCTATGGGAGAGCATAGACATCGAGTATGGGA4300.1275513987642346No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG4220.1251783494849No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG4200.12458508716506635No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT4160.12339856252539905No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG4130.12250866904564855No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG4010.11894909512664667No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGCTATGGGCGAGCGCTATGA3940.1168726770072289No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGCTATGGGCGAGCGCTATGAG3870.11479625888781111No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTATGGG3870.11479625888781111No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA3810.11301647192831017No Hit
TGGGCGAGCGCTATGAGCGACTATGGGACAGCAGTACTACTGACGATGGG3770.11182994728864289No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT3680.10916026684939147No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACATGGGACTATG3600.10678721757005685No Hit
GTACTGATGACAGCAGTATCTGCTATGGGCGAGCGCTATGAGCGACTATG3550.10530406177047273No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC3510.10411753713080545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAT94050.0279.954251
TGACAGC133700.0279.738958
GTACTGA133500.0279.631841
GGCGATA90300.0279.599671
TGATGAC132850.0279.519385
GATGACA132750.0279.518286
GCGATAC90000.0279.438872
ACTGATG133150.0279.206153
CTGATGA133050.0279.20484
GATACGA90050.0278.815674
GCTATCG93700.0278.45093
ATGACAG133750.0278.268777
CGATACG90550.0278.05193
CTATCGA94100.0277.267244
TACTGAT134350.0277.235232
ATACGAC90700.0276.66265
CGCTATC95250.0275.099732
TATCGAC95150.0274.207555
CGACAGC186450.0273.690558
TACGACA92000.0272.29516
Produced by FastQC (version 0.11.5)