FastQCFastQC Report
Fri 11 Sep 2020
000000000-G69NV_l01_n02_JRS_018_C8C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NV_l01_n02_JRS_018_C8C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914196
Sequences flagged as poor quality0
Sequence length287
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT12175213.31793182206004No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT11309212.370651370165698No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT10656911.657128230707638No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG53220.5821508735544675No Hit
GACAGCAGTATCATCGACAGCAGTGTGTGTTGTTGTTTTGTTGTTAACTT40280.44060573443769174No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG38290.4188379734761474No Hit
GACAGCAGTATCATCGAATAATAAAACTTAATATCTATTCAAATTAATAA35570.3890850539709209No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC34350.37573999448695905No Hit
GACAGCAGTATCATCGAGAGACAGTATGGAGTCGAGAGCGCGCTCGTGGA28050.3068269823976478No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG25280.2765271342250458No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACGTGGTG23330.25519691619740187No Hit
GACAGCAGTATCATTGACTCTATTCAAATTAATAAATAAACCTCGATATA22210.24294571404819099No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC21990.24053922791173887No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAT21760.23802335604181157No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC21570.23594502710578474No Hit
GACAGCAGTATCATCTATGGAGTCGTCAGCAGTACTACTGAGTACTACTA20650.22588153962607582No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG20620.2255533824256505No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG20040.21920900988409486No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG18140.1984257205238264No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGA17490.19131564784794508No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGATCGACAAATGGCAGTATT17480.19120626211446998No Hit
GACAGCAGTATCATCGACAATGGATCGACAAATGGCAGTATTCATCCACA17410.1904405619801443No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGCG16780.18354926077121317No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAGTATGGATC16320.1785175170313587No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT15040.16451614314654625No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG14780.16167211407619372No Hit
GACAGCAGTATCATCGATGGATACGATCGACAAATGGCAGTATTCATCCA14470.15828115633846573No Hit
GTACTGATGACAGCAGTATCATCATATAAATAAATAAACATAAATAAATT14410.15762484193761514No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACATCCTATG14280.15620282740243888No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA14180.1551089700676879No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC13780.150733540728684No Hit
GACAGCAGTACTCCACATACATTATACGAAGTTATGCTAGCCTAGTTCCA13660.14942091192698284No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGTCATGGGCGAGCGC13580.14854582605918204No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGAGCAGTGCGA12760.13957619591422407No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG12740.1393574244472739No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCCTATGGGCGAGCG12700.1389198815133735No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGATCATCAGCATGGATCGA12170.13312243763919335No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG11990.1311534944366416No Hit
GTACTGATGACAGCAGTATCTGCTGTATCCATGTGGCCATACAGTTGTAA11990.1311534944366416No Hit
GACAGCAGTATCATCGAGCGATGATCGTGGATCATGGATCGACAAATGGC11500.12579359349636182No Hit
GACAGCAGTATCATCGAGTCGATCATGTATCATCGATGCTAGCCTAGTTC11040.12076184975650735No Hit
GACAGCAGTATCATCGAACCTCCCACACCTCCCCCTGAACCTGAAACATA10790.11802720641962994No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGAGAGCGCGCT10690.11693334908487896No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG10670.11671457761792876No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG10650.11649580615097856No Hit
GTACTGATGACAGCGCGCTGGGACAGAGATATCATGCGGTATGGATCGAC10220.11179221961154938No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACATATGGGA10170.1112452909441739No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAATTCCACCA10120.11069836227679841No Hit
GACAGCAGTATCATCGCGAGCGCTATGGATCGACAAATGGCAGTATTCAT10110.11058897654332332No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGC9580.10479153266914316No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACACTATGGGCGA9490.10380706106786729No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCTATGGGCGAGCGC9470.10358828960091708No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACAGCAGTATCATCGAGC9450.1033695181339669No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATA9310.10183811786531552No Hit
GACAGCAGTATCATCGAAATAAAGCATTTTTTTCACTGCATTCTAGTTGT9300.10172873213184043No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC9240.10107241773098985No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGAGTATGGGAC9190.10052548906361437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC164950.0280.148222
GGCGATA166300.0279.563751
TGATGAC238300.0279.29025
CCGCTAT202000.0279.19161
GCTATCG199300.0278.95563
GATGACA239100.0278.708286
TGACAGC235850.0278.319278
CTATCGA200050.0277.90984
GTACTGA242800.0276.48641
TATCGAC201800.0274.664285
CGCTATC203450.0273.956022
ATGACAG244650.0272.730227
CGATACG171250.0272.057223
TACTGAT244850.0271.76152
GATACGA172400.0270.812934
TACGACA170500.0269.875376
ATACGAC172400.0268.531045
GCTAACG1200.0234.166693
ACTGATG293700.0227.66073
CGACAGC447750.0227.184818
Produced by FastQC (version 0.11.5)