FastQCFastQC Report
Fri 11 Sep 2020
000000000-G69NV_l01_n02_JRS_018_C8A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NV_l01_n02_JRS_018_C8A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences881777
Sequences flagged as poor quality0
Sequence length287
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT20243522.957618536205864No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT16372418.56750629694356No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT14885716.881479104127234No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC35270.39998775200532566No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG29120.3302422267761577No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAACTATGG28880.32752045018184867No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG27020.30642668157595404No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT25180.28555972768625176No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACTATGGGCGAGC24670.27977595242334513No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC24580.2787552862004793No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT23330.26457936643845326No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACACTATGGG20020.2270415309086084No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA19200.21774212754471933No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC18290.20742205795796442No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCAATTTTCTCTTAA18260.2070818358836758No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCTATGG18220.20662820645129099No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC18000.2041332445731744No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG17540.19891650610074885No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG16370.18564784520349248No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTACAGTCGCTACGACT16150.1831528833253759No Hit
CCGCTATCGACAGCAGTATCTGCATGGGTACAGCAGTATCTATGGGACAG15920.18054451408916314No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG15560.17646184919769964No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG14530.16478089131379023No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCCGAG14490.1643272618814054No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCTA14000.1587703013346912No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA13130.1489038611803211No Hit
CCGCTATCGACAGCAGCTATGGGAGAGCGCGCTCGTCGACATGCTAGAGT12950.14686252873458935No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12630.14323349327551071No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGGGAG12570.14255304912693345No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTATGGGCGAGCGCTAT12120.13744971801260408No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGCAT12090.13710949593831548No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGAGAGGG11700.13268660897256335No Hit
GTACTGATGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC11460.12996483237825435No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG11360.12883075879729228No Hit
GTACTGATGACAGCAGTATCTATGGGCGAGCGCTATGAGCGACTATGGGA11340.12860394408109987No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAAC10830.12282016881819326No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACCTATGGGC10800.12247994674390464No Hit
GGCGATACGACAGCAGTATCTGGATGGATCAGTATGGATCGACAAATGGC10770.12213972466961602No Hit
GTACTGATGACAGCAGTATCTGCTATGGGAGAGCGCGCTCGTCGACTATG10750.12191290995342359No Hit
GGCGATACGACAGCAGTATCTGCTGCTATGGGACAGAGATATCATGCAAG10320.11703639355528665No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACAGCACGACAGTCGACT9800.11113921093428383No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGCGCTAT9420.10682973132662794No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCAGT9390.10648950925233933No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGCG9240.1047883988808962No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCGCT9140.10365432529993411No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGCGCT9060.10274706643516444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA218800.0280.422061
CCGCTAT235050.0280.282681
TGACAGC302200.0280.256138
TGATGAC302250.0280.11685
GATGACA302550.0279.93196
GCGATAC219600.0279.528472
GTACTGA306300.0278.339541
ACTGATG305200.0277.961673
GCTATCG235750.0277.722173
ATGACAG305550.0277.183447
CTATCGA236200.0276.776674
TACTGAT307100.0276.150452
GATACGA222500.0276.137764
CGCTATC238700.0275.879122
TCGACAG235750.0275.874637
CGATACG222750.0275.575533
CTGATGA308150.0275.346284
CGACAGC460600.0274.716258
ATACGAC223500.0274.27365
TATCGAC237750.0274.085825
Produced by FastQC (version 0.11.5)