FastQCFastQC Report
Fri 11 Sep 2020
000000000-G69NV_l01_n01_JRS_018_C9T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NV_l01_n01_JRS_018_C9T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307854
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGAGC403.6010697E-622.0000027
CCACAGA403.6010697E-622.0000025
AGCAAAT353.2053264E-522.08
GGCAGGC250.00257611222.06
GTCGCGA250.00257611222.01
AGATCCG605.9299055E-1022.05
GTGGGCG250.00257611222.09
ACGGGCG250.00257611222.02
TCAGCTT504.5958586E-822.06
AATTCTT250.00257611222.022
AACCTCT750.022.02
CCGAATC302.8660483E-422.03
GCAGCTA353.2053264E-522.010
CTGATCG250.00257611222.011
TCGAACG250.00257611222.08
GGCTAGC302.8660483E-422.04
CCGGGTA454.0614032E-721.9999981
AGTGCCG454.0614032E-721.9999981
TACCGGG454.0614032E-721.9999981
CGTCGGA1500.021.2666666
Produced by FastQC (version 0.11.5)