FastQCFastQC Report
Fri 11 Sep 2020
000000000-G69NV_l01_n01_JRS_018_C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NV_l01_n01_JRS_018_C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591700
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAGA750.022.05
GTGAGGG250.002578896222.011
ACGCACG3450.021.043481
CGGTGTC3400.021.0294136
GGACACG2050.020.9268282
GGACAGG1000.020.910
AGCCGAA3600.020.7777798
GTGCCTC900.020.77777910
GGCGTAA3400.020.70588310
ATTAGTC3350.020.6865673
GGGGTGA1600.020.6250025
GACGGAG801.8189894E-1220.6250023
AGTAATA801.8189894E-1220.6250028
GGATAGC2300.020.5652184
ACGGGAG701.8371793E-1020.4285723
AATCGGG2650.020.33962210
ACACCCC1300.020.3076932
CGGAGCC651.6189006E-920.3076935
CACCCCA1300.020.3076933
TACCGGG1850.020.2162171