FastQCFastQC Report
Fri 11 Sep 2020
000000000-G69NV_l01_n01_JRS_018_C8G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G69NV_l01_n01_JRS_018_C8G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337119
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAGA250.002576614522.04
CGGTTCC250.002576614522.06
AAAAGAT353.2064934E-522.05
ACGGGAC250.002576614522.02
CGGGAGG302.8668318E-422.04
GGGCAAC302.8668318E-422.05
GGCTCGG302.8668318E-422.05
TAGATGC752.0008883E-1120.5333337
CGGACAC601.4222678E-820.1666661
GGATAGT551.258868E-720.03
CCAGATC551.258868E-720.03
GCCTGTG551.258868E-720.04
GGGATCG551.258868E-720.04
GCACCAC501.1185202E-619.8000017
ATGCCTG501.1185202E-619.8000012
TATAAGG501.1185202E-619.8000019
GGGAGGG459.98452E-619.5555555
GTGGAGG459.98452E-619.5555558
TGCACCA459.98452E-619.5555556
ATCCTTG408.9640984E-519.259
Produced by FastQC (version 0.11.5)