FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_TRAF5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_TRAF5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8743
Sequences flagged as poor quality0
Sequence length300
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAATTCCGGCAA758186.70936749399519No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAATCCGGCAAC3173.625757749056388No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGATTCCGGCAAC1391.5898433032139998No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAATTCCGGCAAC670.7663273475923595No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGGAATTCCGGCAAC310.3545693697815395No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGATCCGGCAACT300.34313164817568337No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAATCCGGCAACT290.33169392656982727No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACCCGGAATTCCGGCAAC250.2859430401464028No Hit
ATCGATTCCTTGGTTCTGTGGAAAGGACGAAACACCGGAATTCCGGCAAC210.24019215372297836No Hit
ATCGATTCCTTGGTTCTTGTGAAAGGACGAAACACCGGAATTCCGGCAAC180.20587898890541004No Hit
ATCGATTCCTTGTTCTTGTGGAAAGGACGAAACACCGGAATTCCGGCAAC150.17156582408784168No Hit
ATCGATTCTTGGTTCTTGTGGAAAGGACGAAACACCGGAATTCCGGCAAC150.17156582408784168No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACCCGGAATCCGGCAACT120.13725265927027336No Hit
ATCGATTCCTTGGTTTTGTGGAAAGGACGAAACACCGGAATTCCGGCAAC110.12581493766441726No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAACACCGGAATTCCGGCAAC110.12581493766441726No Hit
ATCGATTCCTTGGTCTTGTGGAAAGGACGAAACACCGGAATTCCGGCAAC110.12581493766441726No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGGAACACCGGAATTCCGGCAA100.11437721605856113No Hit
ATCGATTCCTTGGTTCTTGTGGAAAAGACGAAACACCGGAATTCCGGCAA90.10293949445270502No Hit
ATCGATTCCTTGGTTCTTGGGAAAGGACGAAACACCGGAATTCCGGCAAC90.10293949445270502No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGACGAAACACCGGAATTCCGGCAAC90.10293949445270502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)