FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_TRAF5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_TRAF5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16015
Sequences flagged as poor quality0
Sequence length300
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGT1464491.4392756790509No Hit
CGATCATGATCGTATTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGT1390.8679363097096472No Hit
CGATCATGATAGTCTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGT1140.7118326568841711No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGGTTGTTCAGTGTT670.418357789572276No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACGGGTTGTTTCAGTGTT630.39338120512019986No Hit
CGATCATGATCGTCTTGTGGAAGGACGAAACACCGGGTTGTTTCAGTGTT620.38713705900718076No Hit
CGATCATGATCGTCTTGTGAAAGGACGAAACACCGGGTTGTTTCAGTGTT550.34342803621604745No Hit
CGATCATGATCGTCTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT450.280986575085857No Hit
CGATCATGATCGTCTTGTGGAAAGACGAAACACCGGGTTGTTTCAGTGTT340.21230096784264751No Hit
CGATCATGATCGTCTTTGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT310.19356852950359038No Hit
CGATCATGATCGTCTTGGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT250.1561036528254761No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGTTGTTTCAGTGTT210.13112706837339994No Hit
CGATCATGATCGTTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT210.13112706837339994No Hit
CGATCATGATGTCTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT200.1248829222603809No Hit
CGATCATGAGCGTCTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGT200.1248829222603809No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)