FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_TRAF3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_TRAF3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24952
Sequences flagged as poor quality0
Sequence length300
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAATGGTCT2324193.14283424174414No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAGGACGAAACACCGGAATGGTCTG1290.5169926258416159No Hit
ACGATCGATAGGTAAGGTCTGTGGAAAGGACGAAACACCGGAATGGTCTG800.3206155819172812No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAGGACGAAACACCGGAATGGTCTG590.23645399166399486No Hit
ACGATCGATAGGTAGGTCTTGTGGAAAGGACGAAACACCGGAATGGTCTG570.22843860211606287No Hit
ACGATCGATAGGTAAGTCTTGTGGAAAGGACGAAACACCGGAATGGTCTG510.20439243347226674No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAATGGTCTG510.20439243347226674No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAATGTCTG400.1603077909586406No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATGGTCTG390.15630009618467458No Hit
ACGATCGATAGGTAAGGTTTGTGGAAAGGACGAAACACCGGAATGGTCTG370.14828470663674254No Hit
ACGATCGATAGTAAGGTCTTGTGGAAAGGACGAAACACCGGAATGGTCTG360.14427701186277653No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGACGAAACACCGGAATGGTCTG300.12023084321898043No Hit
ACGATCGATGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAATGGTCTG290.11622314844501441No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGGCGAAACACCGGAATGGTCT280.11221545367104842No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)