FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_TRAF2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_TRAF2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18527
Sequences flagged as poor quality0
Sequence length300
%GC59

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGCTCCCTGGAGTTGC1539783.10573757219194No Hit
GATCGCGCGTTTCTTGTGGAAAGGACGAAACACCGGCTCCCTGGAGTTGC17589.488854104819993No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGCTCCCTGGAGTGCT700.37782695525449345No Hit
GATCGCGCGGTTCTGTGGAAAGGACGAAACACCGGCTCCCTGGAGTTGCT620.33464673179683707No Hit
GATCGCGCGGTTCTTGTGGAAGGACGAAACACCGGCTCCCTGGAGTTGCT610.32924920386462997No Hit
GATCGCGCGGTTCTTGTGAAAGGACGAAACACCGGCTCCCTGGAGTTGCT440.23749122901711017No Hit
GATCGCGCGGTTCTTTGGAAAGGACGAAACACCGGCTCCCTGGAGTTGCT330.17811842176283263No Hit
GATCGCGCGGTTCTTGGGAAAGGACGAAACACCGGCTCCCTGGAGTTGCT330.17811842176283263No Hit
GATCGCGCGTTCTTGTGGAAAGGACGAAACACCGGCTCCCTGGAGTTGCT300.1619258379662115No Hit
GATCGCGCGGTTTTGTGGAAAGGACGAAACACCGGCTCCCTGGAGTTGCT280.15113078210179737No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAACACCGGCTCCCTGGAGTTGCT270.14573325416959032No Hit
GATCGCGCGGTCTTGTGGAAAGGACGAAACACCGGCTCCCTGGAGTTGCT270.14573325416959032No Hit
GATCGCGCGGTTCTTGTGGAAAGACGAAACACCGGCTCCCTGGAGTTGCT220.11874561450855509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)