FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_TRAF2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_TRAF2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22785
Sequences flagged as poor quality0
Sequence length300
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCAGGTCATGTAACCTC2126893.34211103796358No Hit
ATACACGATCTCTGTGGAAAGGACGAAACACCGCAGGTCATGTAACCTCT1020.4476629361421988No Hit
ATACACGATCTCTTGTGGAAGGACGAAACACCGCAGGTCATGTAACCTCT820.3598858898398069No Hit
ATACACGATCTCTTGTGAAAGGACGAAACACCGCAGGTCATGTAACCTCT710.31160851437349135No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCAGGTCATGTACCTCT450.19749835418038184No Hit
ATACACGATCTCTTTGGAAAGGACGAAACACCGCAGGTCATGTAACCTCT410.17994294491990345No Hit
ATACACGATCTCTTGTGGAAAGACGAAACACCGCAGGTCATGTAACCTCT390.17116524028966426No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCAGTCATGTAACCTCT380.16677638797454464No Hit
ATACACGATCTCTTGGGAAAGGACGAAACACCGCAGGTCATGTAACCTCT380.16677638797454464No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCAGGTCATGTAACTCT310.13605442176870747No Hit
ATACACGATTCTTGTGGAAAGGACGAAACACCGCAGGTCATGTAACCTCT280.1228878648233487No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCAGGTATGTAACCTCT280.1228878648233487No Hit
ATACACGATCTTTGTGGAAAGGACGAAACACCGCAGGTCATGTAACCTCT280.1228878648233487No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACGCAGGTCATGTAACCTCT250.10972130787798992No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCAGGTCTGTAACCTCT240.1053324555628703No Hit
ATACACGATCTCTTGTAGAAAGGACGAAACACCGCAGGTCATGTAACCTC240.1053324555628703No Hit
ATACACGATCTCTTGTGGAAAGGACGAACACCGCAGGTCATGTAACCTCT240.1053324555628703No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGCGGTCATGTAACCTCT230.1009436032477507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)