FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_RELB_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_RELB_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18456
Sequences flagged as poor quality0
Sequence length300
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGATGTCCTGGCTTAGC1723693.38968357173819No Hit
ATACACGATCTCTGTGGAAAGGACGAAACACCGGATGTCCTGGCTTAGCT750.4063719115734721No Hit
ATACACGATCTCTTGTGGAAGGACGAAACACCGGATGTCCTGGCTTAGCT610.3305158214130906No Hit
ATACACGATCTCTTGTGAAAGGACGAAACACCGGATGTCCTGGCTTAGCT590.31967923710446466No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACGGATGTCCTGGCTTAGCT480.26007802340702213No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGATGTCCTGGCTTAGCT400.21673168617251842No Hit
ATACACGATCTCTTTGGAAAGGACGAAACACCGGATGTCCTGGCTTAGCT370.20047680970957954No Hit
ATACACGATCTCTTGTGGAAAGACGAAACACCGGATGTCCTGGCTTAGCT320.17338534893801474No Hit
ATACACGATCTTTGTGGAAAGGACGAAACACCGGATGTCCTGGCTTAGCT300.1625487646293888No Hit
ATACACGATTCTTGTGGAAAGGACGAAACACCGGATGTCCTGGCTTAGCT250.135457303857824No Hit
ATACACGATCTCTTGGGAAAGGACGAAACACCGGATGTCCTGGCTTAGCT240.13003901170351106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)