FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_RELB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_RELB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21156
Sequences flagged as poor quality0
Sequence length300
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTCTCCACTTCCCACCCT1968393.03743618831537No Hit
TAAGTAGAGTATTGTGGAAAGGACGAAACACCGTCTCCACTTCCCACCCT1280.6050293061070146No Hit
TAAGTAGAGGCTTGTGGAAAGGACGAAACACCGTCTCCACTTCCCACCCT650.3072414445074683No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTCTCCACTTCCACCCTC570.2694271128757799No Hit
TAAGTAGAGTCTTGTGGAAGGACGAAACACCGTCTCCACTTCCCACCCTC480.22688598979013047No Hit
TAAGTAGAGTCTTGTGAAAGGACGAAACACCGTCTCCACTTCCCACCCTC450.2127056154282473No Hit
TAAGTAGAGTCTGTGGAAAGGACGAAACACCGTCTCCACTTCCCACCCTC430.20325203252032523No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTCTCACTTCCCACCCTC380.17961807525051995No Hit
TAAGTAGAGTCTTGGGAAAGGACGAAACACCGTCTCCACTTCCCACCCTC300.14180374361883152No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAACACCGTCTCCACTTCCCACCCTC230.10871620344110418No Hit
TAAGTAGAGTCTTTGGAAAGGACGAAACACCGTCTCCACTTCCCACCCTC220.10398941198714313No Hit
TAAGTAGAGTCTTGTGGAAAGACGAAACACCGTCTCCACTTCCCACCCTC220.10398941198714313No Hit
TAAGTAGAGTTTGTGGAAAGGACGAAACACCGTCTCCACTTCCCACCCTC220.10398941198714313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)