FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_RELA_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_RELA_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23712
Sequences flagged as poor quality0
Sequence length300
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTA2206093.03306342780027No Hit
TAACAATGGTATTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTA1730.7295883940620783No Hit
TAACAATGGGCTTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTA1160.4892037786774629No Hit
TAACAATGGTCTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTAT950.4006410256410256No Hit
TAACAATGTCTTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTAT700.2952091767881242No Hit
TAACAATGGTCTTGTGGAAGGACGAAACACCGCCCACTGATAGCACCTAT670.282557354925776No Hit
TAACAATGGTCTTGTGAAAGGACGAAACACCGCCCACTGATAGCACCTAT630.2656882591093117No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCCACTGATAGCACTAT430.18134278002699056No Hit
TAACAATGGTCTTGTGGAAAGACGAAACACCGCCCACTGATAGCACCTAT370.15603913630229418No Hit
TAACAATGGTCTTGGGAAAGGACGAAACACCGCCCACTGATAGCACCTAT360.15182186234817813No Hit
TAACAATGGTCTTTGGAAAGGACGAAACACCGCCCACTGATAGCACCTAT340.14338731443994604No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTGATAGCACCTAT250.10543184885290148No Hit
TAACAATGGTTTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTAT250.10543184885290148No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)