FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_NFKB2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_NFKB2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22782
Sequences flagged as poor quality0
Sequence length300
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTT2106192.44579053638837No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAGGACGAAACACCGGGCTTCCTTG1050.4608901764550961No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAGGACGAAACACCGGGCTTCCTTG650.2853129663769643No Hit
ACGATCGATAGGTAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG620.27214467562110434No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCTTCCTTG580.25458695461329117No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTG570.2501975243613379No Hit
ACGATCGATAGGTAAGGTCTGTGGAAAGGACGAAACACCGGGCTTCCTTG560.24580809410938462No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCTTG540.23702923360547798No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGACGAAACACCGGGCTTCCTTG450.19752436133789833No Hit
ACGATCGATAGGTAAGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG420.18435607058203846No Hit
ACGATCGATAGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG330.14485119831445878No Hit
ACGATCGATGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG290.12729347730664559No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACGGGCTTCCTTG280.12290404705469231No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAACACCGGGCTTCCTTG280.12290404705469231No Hit
ACGATCGATAGGTAAGGTCTTGGGAAAGGACGAAACACCGGGCTTCCTTG280.12290404705469231No Hit
ACGATCGATAGGTAAGGTCTTTGGAAAGGACGAAACACCGGGCTTCCTTG270.11851461680273899No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTCCTTG240.10534632604687912No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)