FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_NFKB2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_NFKB2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17001
Sequences flagged as poor quality0
Sequence length300
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTA1535790.32998058937709No Hit
CGATCGATACCGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTA3782.2233986236103758No Hit
CGATCGATACAGGTATTCTTGTGGAAGGACGAAACACCGCAGCTTCTTAA660.38821245809070054No Hit
CGATCGATACAGGTATTCTGTGGAAAGGACGAAACACCGCAGCTTCTTAA610.3588024233868596No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGTTCTTAA440.25880830539380034No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTTTAA390.22939827068995938No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTCTTAA290.1705782012822775No Hit
CGATCGATACAGGTATTTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA270.15881418740074113No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTAA270.15881418740074113No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCTTCTTAA260.15293218045997295No Hit
CGATCGATACAGGTATCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA230.13528615963766835No Hit
CGATCGATACAGGTATTCTTGTGGAAAGACGAAACACCGCAGCTTCTTAA230.13528615963766835No Hit
CGATCGATACAGGTATTCTTGTGAAAGGACGAAACACCGCAGCTTCTTAA210.12352214575613199No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACGCAGCTTCTTAA210.12352214575613199No Hit
CGATCGATACAGGAATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTA210.12352214575613199No Hit
CGATCGATACAGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA200.1176401388153638No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)