FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_NFKB2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_NFKB2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20272
Sequences flagged as poor quality0
Sequence length300
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCCA1867792.1320047355959No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACTATCCCAC860.4242304656669298No Hit
GATCGATAACGCATTTCTTGTGGAAGGACGAAACACCGGACCTATCCCAC810.39956590370955014No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCAC740.3650355169692186No Hit
GATCGATAACGCATTTCTGTGGAAAGGACGAAACACCGGACCTATCCCAC620.3058405682715075No Hit
GATCGATAACGCATTTCTTGTGAAAGGACGAAACACCGGACCTATCCCAC460.22691397000789265No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCAA410.202249408050513No Hit
GATCGATAACGCATTCTTGTGGAAAGGACGAAACACCGGACCTATCCCAC310.15292028413575376No Hit
GATCGATAACGATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCCAC300.1479873717442778No Hit
GATCGATACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCCAC280.13812154696132595No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCCC270.13318863456985006No Hit
GATCGATAACGCATTTCTTGTGGAAAGACGAAACACCGGACCTATCCCAC270.13318863456985006No Hit
GATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA270.13318863456985006No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTTCCCAC260.1282557221783741No Hit
GATCGATAACGCATTTTTGTGGAAAGGACGAAACACCGGACCTATCCCAC250.12332280978689819No Hit
GATCGATAACGCATTTATTGTGGAAAGGACGAAACACCGGACCTATCCCA240.11838989739542227No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGGACCTATCCC240.11838989739542227No Hit
GATCGATAACGCATTTCTTTGGAAAGGACGAAACACCGGACCTATCCCAC240.11838989739542227No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACGGACCTATCCCAC230.11345698500394633No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAACACCGGACCTATCCCAC220.1085240726124704No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGCCTATCCCAC220.1085240726124704No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCATCCCAC210.10359116022099447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)