FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_NFKB1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_NFKB1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20546
Sequences flagged as poor quality0
Sequence length300
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCG1683481.93322301177844No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGATGTCCTGGCT214610.444855446315584No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGGAGAGGATTTCGT950.4623771050326098No Hit
ATCGATTCCTTGGTTCTTGTGAAAGGACGAAACACCGGAGAGGATTTCGT510.2482234984911905No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGACGAAACACCGGAGAGGATTTCGT380.1849508420130439No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAGAGGATTTCGT370.18008371459164801No Hit
ATCGATTCCTTGGTTCTTGGGAAAGGACGAAACACCGGAGAGGATTTCGT340.16548233232746035No Hit
ATCGATTCCTTGGTTCTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT330.16061520490606446No Hit
ATCGATTCCTTGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT330.16061520490606446No Hit
ATCGATTCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT310.15088095006327265No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGGAGGATTTCGT280.13627956779908498No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGATTTCGT280.13627956779908498No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAGAGGATTTCGT250.1216781855348973No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTAG240.1168110581135014No Hit
ATCGATTCCGTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCG230.11194393069210552No Hit
ATCGATTCCTTGGTTTTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT220.10707680327070963No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTCGT220.10707680327070963No Hit
ATCGATTCCTTGGTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT210.10220967584931373No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCT210.10220967584931373No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)