FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_MAP3K5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_MAP3K5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24643
Sequences flagged as poor quality0
Sequence length300
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTA2175488.27658970092928No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTT7072.8689688755427505No Hit
TAACAATGGTATTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTA2641.071298137402102No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATCA2040.8278212879925334No Hit
TAACAATGGTCTTGTGGAAGGACGAAACACCGGTGCTGATTGGGCATTAT1060.43014243395690466No Hit
TAACAATGGTCTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT950.3855050115651504No Hit
TAACAATGGGCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTA900.36521527411435295No Hit
TAACAATGGTCTTGTGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT610.2475347968997281No Hit
TAACAATGTCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT490.19883942701781437No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTGCTGATTGGGCATTAT450.18260763705717648No Hit
TAACAATGGTCTTGTGGAAAGACGAAACACCGGTGCTGATTGGGCATTAT440.178549689567017No Hit
TAACAATGGTCTTTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT410.16637584709653855No Hit
TAACAATGGTTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT370.15014405713590068No Hit
TAACAATGGTCTTGGGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT370.15014405713590068No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACGGTGCTGATTGGGCATTAT290.11768047721462485No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGCATTAT290.11768047721462485No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)