FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_MAP3K14_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_MAP3K14_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8491
Sequences flagged as poor quality0
Sequence length300
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAG795493.6756565775527No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGCTGTGTAGACAGC560.6595218466611706No Hit
ATCGATTCCTTGGTTCTTGTGAAAGGACGAAACACCGCTGTGTAGACAGC200.2355435166647038No Hit
ATCGATTCCTTGGTTCTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC200.2355435166647038No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGACGAAACACCGCTGTGTAGACAGC160.18843481333176304No Hit
ATCGATTCTTGGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC160.18843481333176304No Hit
ATCGATTCCTTGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC110.12954893416558708No Hit
ATCGATTCCGTGGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAG110.12954893416558708No Hit
ATCGATTCCTGGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC110.12954893416558708No Hit
ATCGATTCCTTGGTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC90.1059945824991167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)