FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_MAP3K14_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_MAP3K14_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16484
Sequences flagged as poor quality0
Sequence length300
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCTGGGATTTGAC1514891.89517107498179No Hit
TCGATCGTTAACATCTTGTGGAAAGGACGAAACACCGCCTGGGATTTGAC1851.1223004125212326No Hit
TCGATCGTTACCATCTGTGGAAAGGACGAAACACCGCCTGGGATTTGACC840.5095850521718029No Hit
TCGATCGTTACATCTTGTGGAAAGGACGAAACACCGCCTGGGATTTGACC690.4185877214268382No Hit
TCGATCGTTACCATCTTGTGGAAGGACGAAACACCGCCTGGGATTTGACC670.4064547439941762No Hit
TCGATCGTTACCATCTTGTGAAAGGACGAAACACCGCCTGGGATTTGACC520.31545741324921134No Hit
TCGATCGTTACCATCTTGGGAAAGGACGAAACACCGCCTGGGATTTGACC470.2851249696675564No Hit
TCGATCGTTACCATTTGTGGAAAGGACGAAACACCGCCTGGGATTTGACC340.20626061635525358No Hit
TCGATCGTTACCATCTTGTGGAAAGACGAAACACCGCCTGGGATTTGACC290.17592817277359865No Hit
TCGATCGTACCATCTTGTGGAAAGGACGAAACACCGCCTGGGATTTGACC290.17592817277359865No Hit
TCGATCGTTACCATCTTTGGAAAGGACGAAACACCGCCTGGGATTTGACC280.16986168405726765No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACGCCTGGGATTTGACC270.16379519534093667No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCTGGGATTTGACC220.13346275175928174No Hit
TCGATCGTTACCTCTTGTGGAAAGGACGAAACACCGCCTGGGATTTGACC210.12739626304295074No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGCCTGGATTTGACC190.11526328561028876No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAACACCGCCTGGGATTTGACC180.10919679689395778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)