FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_MAP3K14_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_MAP3K14_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12644
Sequences flagged as poor quality0
Sequence length300
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA1146490.66751028155647No Hit
CGATCATGATAGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA1110.877886744701044No Hit
CGATCATGATCGTATTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA1090.8620689655172413No Hit
CGATCATGATCGTCTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA510.4033533691869661No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAGCCAAGGAGAA480.37962670041126223No Hit
CGATCATGATCGTCTTGTGAAAGGACGAAACACCGCAAAGCCAAGGAGAA420.33217336285985444No Hit
CGATCATGATCGTCTTGTGGAAGGACGAAACACCGCAAAGCCAAGGAGAA360.2847200253084467No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCACAGCCAGAACAC330.2609933565327428No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAGGAGAA320.2530844669408415No Hit
CGATCATGATGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA320.2530844669408415No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCAAGGAGAA260.2056311293894337No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAGCCAAGGAGAA240.18981335020563112No Hit
CGATCATGATCGTTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA240.18981335020563112No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACGCAAAGCCAAGGAGAA240.18981335020563112No Hit
CGATCATGATCGTCTTTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA220.17399557102182853No Hit
CGATCATGATCGTCTTGGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA200.15817779183802594No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGAGAA190.15026890224612463No Hit
CGATCATGATCGTCTTGTGGAAAGACGAAACACCGCAAAGCCAAGGAGAA170.13445112306232204No Hit
CGATCATGAGCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA140.11072445428661816No Hit
CGATCATGATCTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA140.11072445428661816No Hit
CGATCATGTCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA140.11072445428661816No Hit
CGATCATGATCGCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA140.11072445428661816No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)