FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_LTB_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_LTB_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18332
Sequences flagged as poor quality0
Sequence length300
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGATGGTGCTGGTAGGA1714793.53589351952868No Hit
ATACACGATCTCTGTGGAAAGGACGAAACACCGGATGGTGCTGGTAGGAG780.4254854898538075No Hit
ATACACGATCTCTTGTGGAAGGACGAAACACCGGATGGTGCTGGTAGGAG620.3382064150120009No Hit
ATACACGATCTCTTGTGAAAGGACGAAACACCGGATGGTGCTGGTAGGAG500.27274710888064585No Hit
ATACACGATCTCTTGGGAAAGGACGAAACACCGGATGGTGCTGGTAGGAG360.19637791839406502No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACGGATGGTGCTGGTAGGAG350.1909229762164521No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGATGGTGCTGGTAGGAG300.16364826532838753No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGATGTGCTGGTAGGAG280.15273838097316167No Hit
ATACACGATCTCTTTGGAAAGGACGAAACACCGGATGGTGCTGGTAGGAG270.14728343879554878No Hit
ATACACGATCTTTGTGGAAAGGACGAAACACCGGATGGTGCTGGTAGGAG260.14182849661793584No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGTGGTGCTGGTAGGAG240.13091861226271004No Hit
ATACACGATCTCTTGTGGAAAGGACGAACACCGGATGGTGCTGGTAGGAG240.13091861226271004No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGATGGCGCTGGTAGGA220.12000872790748418No Hit
ATACACGATTCTTGTGGAAAGGACGAAACACCGGATGGTGCTGGTAGGAG220.12000872790748418No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGGATGGTGCTGGTGGAG200.10909884355225835No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)