FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_LTB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_LTB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22656
Sequences flagged as poor quality0
Sequence length300
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGTTCCCTCCTGCTAGCT1779278.53107344632768No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGCAGGTCATGTAACCTCT325114.349399717514125No Hit
TAAGTAGAGTATTGTGGAAAGGACGAAACACCGGTTCCCTCCTGCTAGCT1790.7900776836158191No Hit
TAAGTAGAGGCTTGTGGAAAGGACGAAACACCGGTTCCCTCCTGCTAGCT620.2736581920903955No Hit
TAAGTAGAGTCTGTGGAAAGGACGAAACACCGGTTCCCTCCTGCTAGCTG560.24717514124293788No Hit
TAAGTAGAGTCTTGTGAAAGGACGAAACACCGGTTCCCTCCTGCTAGCTG450.1986228813559322No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGTTCCTCCTGCTAGCTG450.1986228813559322No Hit
TAAGTAGAGTCTTGTGGAAGGACGAAACACCGGTTCCCTCCTGCTAGCTG430.189795197740113No Hit
TAAGTAGAGTCTTTGGAAAGGACGAAACACCGGTTCCCTCCTGCTAGCTG250.11034604519774012No Hit
TAAGTAGAGTCTTGGGAAAGGACGAAACACCGGTTCCCTCCTGCTAGCTG250.11034604519774012No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGTTCCCTCTGCTAGCTG240.1059322033898305No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAACACCGGTTCCCTCCTGCTAGCTG230.1015183615819209No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)