FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_LTA_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_LTA_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14276
Sequences flagged as poor quality0
Sequence length300
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTGAGGCAGCA1297590.88680302605772No Hit
CGATCGATACCGGTATTCTTGTGGAAAGGACGAAACACCGTGAGGCAGCA3092.164471840851779No Hit
CGATCGATACAGGTATTCTTGTGGAAGGACGAAACACCGTGAGGCAGCAG600.4202857943401513No Hit
CGATCGATACAGGTATTCTGTGGAAAGGACGAAACACCGTGAGGCAGCAG400.2801905295601009No Hit
CGATCGATACAGGTATTCTTGTGAAAGGACGAAACACCGTGAGGCAGCAG370.2591762398430933No Hit
CGATCGATACAGGTATTCTTGTGGAAAGACGAAACACCGTGAGGCAGCAG210.14710002801905295No Hit
CGATCGATACAGGTATTTTGTGGAAAGGACGAAACACCGTGAGGCAGCAG200.14009526478005044No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTGAGCAGCAG190.13309050154104793No Hit
CGATCGATACAGGTATTCTTGTGGAAAAGACGAAACACCGTGAGGCAGCA180.1260857383020454No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACGTGAGGCAGCAG180.1260857383020454No Hit
CGATCGATACAGGTATCTTGTGGAAAGGACGAAACACCGTGAGGCAGCAG170.11908097506304285No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTGAGGCAGAA170.11908097506304285No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)