FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_LTA_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_LTA_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13231
Sequences flagged as poor quality0
Sequence length300
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGCTCACTGTCT1232193.12221298465725No Hit
GATCGATAACGCATTTCTTGTGGAAGGACGAAACACCGGCTCACTGTCTC600.4534804625500718No Hit
GATCGATAACGCATTTCTGTGGAAAGGACGAAACACCGGCTCACTGTCTC450.3401103469125539No Hit
GATCGATAACGCATTTCTTGTGAAAGGACGAAACACCGGCTCACTGTCTC230.17383417731086087No Hit
GATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGTGAGGCAGCAG220.16627616960169297No Hit
GATCGATAACGCATTCTTGTGGAAAGGACGAAACACCGGCTCACTGTCTC200.15116015418335726No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGCTCACTGTAT190.1436021464741894No Hit
GATCGATAACGCATTTCTTGTGGAAAGACGAAACACCGGCTCACTGTCTC160.12092812334668582No Hit
GATCGATAAAGCATTTCTTGTGGAAAGGACGAAACACCGGCTCACTGTCT140.1058121079283501No Hit
GATCGATAACGCATTTCTTTGGAAAGGACGAAACACCGGCTCACTGTCTC140.1058121079283501No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGCTCACTGTTC140.1058121079283501No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)