FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_LIGHT_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_LIGHT_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13367
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTGGCTATG1244193.07249195780653No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAGGACGAAACACCGGTGGCTATGG650.48627216278895785No Hit
ACGATCGATAGGTAGGTCTTGTGGAAAGGACGAAACACCGGTGGCTATGG470.3516121792474003No Hit
ACGATCGATAGGTAAGGTCTGTGGAAAGGACGAAACACCGGTGGCTATGG450.3366499588538939No Hit
ACGATCGATAGGTAAGTCTTGTGGAAAGGACGAAACACCGGTGGCTATGG320.23939552629610233No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAGGACGAAACACCGGTGGCTATGG270.20198997531233634No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACGGTGGCTATGG220.16458442432857037No Hit
ACGATCGATAGTAAGGTCTTGTGGAAAGGACGAAACACCGGTGGCTATGG210.15710331413181716No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTGGCTATGG210.15710331413181716No Hit
ACGATCGATAGGTAAGGTCTTTGGAAAGGACGAAACACCGGTGGCTATGG180.13465998354155756No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGACGAAACACCGGTGGCTATGG170.12717887334480438No Hit
ACGATCGATAGGTAAGGTCTTGTAGAAAGGACGAAACACCGGTGGCTATG150.11221665295129797No Hit
ACGATCGATAGGTAAGGTCTTGGGAAAGGACGAAACACCGGTGGCTATGG140.10473554275454476No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTGTCTATG140.10473554275454476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)