FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_LIGHT_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_LIGHT_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21382
Sequences flagged as poor quality0
Sequence length300
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTC1950791.23094191375924No Hit
CGATCGATACCGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTC4732.2121410532223367No Hit
CGATCGATACAGGTATTCTTGTGGAAGGACGAAACACCGGAGCAATTTCG1030.4817135908708259No Hit
CGATCGATACAGGTATTCTGTGGAAAGGACGAAACACCGGAGCAATTTCG560.2619025348423908No Hit
CGATCGATACAGGTATTCTTGTGGAAAGACGAAACACCGGAGCAATTTCG360.16836591525582265No Hit
CGATCGATACAGGTATTCTTGTGAAAGGACGAAACACCGGAGCAATTTCG360.16836591525582265No Hit
CGATCGATACAGGTATTTTGTGGAAAGGACGAAACACCGGAGCAATTTCG330.15433542231783742No Hit
CGATCGATACAGGTATCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG320.14965859133850903No Hit
CGATCGATACAGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG290.1356280984005238No Hit
CGATCGATAAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG290.1356280984005238No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTCG280.1309512674211954No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGAATTTCG250.11692077448321019No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCATTTCG250.11692077448321019No Hit
CGATCGATACAGGTATTCTTGGGAAAGGACGAAACACCGGAGCAATTTCG230.10756711252455337No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)