FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_LIGHT_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_LIGHT_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16696
Sequences flagged as poor quality0
Sequence length300
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAA1534691.91423095352181No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAACAAGCAAC1070.640872065165309No Hit
GATCGATAACGCATTTCTTGTGGAAGGACGAAACACCGCAAACAAGCAAC600.3593675131768088No Hit
GATCGATAACGCATTTCTGTGGAAAGGACGAAACACCGCAAACAAGCAAC490.2934834690943939No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAGCAAC470.2815045519885002No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACGCAAACAAGCAAC340.20364159080019165No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAC340.20364159080019165No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAACAAGCAAC330.19765213224724487No Hit
GATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG320.19166267369429804No Hit
GATCGATAACGCATTTCTTGTGAAAGGACGAAACACCGCAAACAAGCAAC320.19166267369429804No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGAAC270.16171538092956397No Hit
GATCGATAACGCATTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAAC260.15572592237661714No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAAAAGCAAC250.14973646382367034No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAACACCGCAAACAAGCAAC230.1377575467177767No Hit
GATCGATAACGATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAAC220.1317680881648299No Hit
GATCGATAACGCATTTCTTTGGAAAGGACGAAACACCGCAAACAAGCAAC210.1257786296118831No Hit
GATCGATAACGCATTTTTGTGGAAAGGACGAAACACCGCAAACAAGCAAC200.11978917105893627No Hit
GATCGATAACGCATTTCTTGTGGAAAGACGAAACACCGCAAACAAGCAAC200.11978917105893627No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACCAAACACCGCAAACAAGCAA170.10182079540009582No Hit
GATCGATAAGGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAA170.10182079540009582No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)