FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_NGFR_JUN_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_NGFR_JUN_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14317
Sequences flagged as poor quality0
Sequence length300
%GC66

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCACAGCCAGAACAC1321392.28888733673256No Hit
CGATCATGATAGTCTTGTGGAAAGGACGAAACACCGCACAGCCAGAACAC990.6914856464343089No Hit
CGATCATGATCGTATTGTGGAAAGGACGAAACACCGCACAGCCAGAACAC860.600684500942935No Hit
CGATCATGATCGTCTTGTGGAAGGACGAAACACCGCACAGCCAGAACACG550.3841586924635049No Hit
CGATCATGATCGTCTTGTGAAAGGACGAAACACCGCACAGCCAGAACACG510.3562198784661591No Hit
CGATCATGATCGTCTGTGGAAAGGACGAAACACCGCACAGCCAGAACACG450.3143116574701404No Hit
CGATCATGATCGTCTTTGGAAAGGACGAAACACCGCACAGCCAGAACACG300.2095411049800936No Hit
CGATCATGATCGTCTTGTGGAAAGACGAAACACCGCACAGCCAGAACACG300.2095411049800936No Hit
CGATCATGATCGTTTGTGGAAAGGACGAAACACCGCACAGCCAGAACACG270.18858699448208424No Hit
CGATCATGATCGTCTTGGGAAAGGACGAAACACCGCACAGCCAGAACACG230.16064818048473842No Hit
CGATCATGAGCGTCTTGTGGAAAGGACGAAACACCGCACAGCCAGAACAC220.15366347698540198No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCACAGCAGAACACG200.13969406998672906No Hit
CGATCATGATGTCTTGTGGAAAGGACGAAACACCGCACAGCCAGAACACG180.12572466298805615No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAACACCGCACAGCCAGAACACG170.1187399594887197No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCACAGCCGAACACG160.11175525598938324No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCACAGCCAGACACG150.1047705524900468No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)