FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_TRAF5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_TRAF5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16710
Sequences flagged as poor quality0
Sequence length300
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGT1527991.43626570915619No Hit
CGATCATGATAGTCTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGT1450.8677438659485339No Hit
CGATCATGATCGTATTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGT1280.7660083782166367No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGGGTTGTTCAGTGTT650.38898862956313585No Hit
CGATCATGATCGTCTTGTGGAAGGACGAAACACCGGGTTGTTTCAGTGTT630.37701974865350085No Hit
CGATCATGATCGTCTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT590.35308198683423103No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACGGGTTGTTTCAGTGTT500.2992220227408737No Hit
CGATCATGATCGTCTTGTGAAAGGACGAAACACCGGGTTGTTTCAGTGTT500.2992220227408737No Hit
CGATCATGATGTCTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT430.2573309395571514No Hit
CGATCATGATCGTCTTTGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT320.19150209455415917No Hit
CGATCATGATCGTTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT310.18551765409934173No Hit
CGATCATGATCGTCTTGGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT310.18551765409934173No Hit
CGATCATGATCGTCTTGTGGAAAGACGAAACACCGGGTTGTTTCAGTGTT310.18551765409934173No Hit
CGATCATGAGCGTCTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGT240.1436265709156194No Hit
CGATCATGTCGTCTTGTGGAAAGGACGAAACACCGGGTTGTTTCAGTGTT170.10173548773189708No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAACACCGGGTTGTTTCAGTGTT170.10173548773189708No Hit
CGATCATGATCGTCTTGTGGAAAAGACGAAACACCGGGTTGTTTCAGTGT170.10173548773189708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)