FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_RELB_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_RELB_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25315
Sequences flagged as poor quality0
Sequence length300
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGGAAGGGCTAGAAGA2029180.15405885838436No Hit
GATCGCGCGTTTCTTGTGGAAAGGACGAAACACCGGGAAGGGCTAGAAGA23399.239581275923365No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGCTCCCTGGAGTTGC2801.106063598656923No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGAAGGGCTAGAAGAG1580.6241358878135493No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGGAGGGCTAGAAGAG1120.44242543946276913No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGCTCACTGTCTCTCT1070.42267430377246695No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGGAAGGCTAGAAGAG880.3476199881493186No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACGGGAAGGGCTAGAAGAG880.3476199881493186No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGGAAGGGCTAGAGAG730.288366581078412No Hit
GATCGCGCGGTTCTTGTGGAAGGACGAAACACCGGGAAGGGCTAGAAGAG640.2528145368358681No Hit
GATCGCGCGGTTCTGTGGAAAGGACGAAACACCGGGAAGGGCTAGAAGAG580.22911317400750542No Hit
GATCGCGCGTTCTTGTGGAAAGGACGAAACACCGGGAAGGGCTAGAAGAG560.22121271973138457No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGGAAGGGCTGAAGAG500.19751135690302193No Hit
GATCGCGCGGTTCTTGTGAAAGGACGAAACACCGGGAAGGGCTAGAAGAG490.19356112976496148No Hit
GATCGCGCGTTTCTTGTGGAAAGGACGAAACACCGGCTCCCTGGAGTTGC400.15800908552241755No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGGAAGGGCAGAAGAG360.1422081769701758No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGGAAGGGCTAAAGAG360.1422081769701758No Hit
GATCGCGCGGTTCTTTGGAAAGGACGAAACACCGGGAAGGGCTAGAAGAG340.13430772269405492No Hit
GATCGCGCGGTTTTGTGGAAAGGACGAAACACCGGGAAGGGCTAGAAGAG330.13035749555599446No Hit
GATCGCGCGGTCTTGTGGAAAGGACGAAACACCGGGAAGGGCTAGAAGAG330.13035749555599446No Hit
GATCGCGCGGTTCTTGGGAAAGGACGAAACACCGGGAAGGGCTAGAAGAG320.12640726841793404No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAACACCGGGAAGGGCTAGAAGAG290.11455658700375271No Hit
GATCGCGCGGTTCTTGTGGAAAGACGAAACACCGGGAAGGGCTAGAAGAG290.11455658700375271No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGGGAAGGGTAGAAGAG280.11060635986569228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)