FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_RELB_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_RELB_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27901
Sequences flagged as poor quality0
Sequence length300
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTCTCCACTTCCCACCCT2390985.69226909429769No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGTTTAAACTGTGGTGTG20587.376079710404645No Hit
TAAGTAGAGTATTGTGGAAAGGACGAAACACCGTCTCCACTTCCCACCCT1380.49460592810293535No Hit
TAAGTAGAGGCTTGTGGAAAGGACGAAACACCGTCTCCACTTCCCACCCT790.2831439733342891No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTCTCCACTTCCACCCTC620.2222142575534927No Hit
TAAGTAGAGTCTGTGGAAAGGACGAAACACCGTCTCCACTTCCCACCCTC620.2222142575534927No Hit
TAAGTAGAGTCTTGTGGAAGGACGAAACACCGTCTCCACTTCCCACCCTC530.18995734919895346No Hit
TAAGTAGAGTCTTGTGAAAGGACGAAACACCGTCTCCACTTCCCACCCTC490.17562094548582488No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAACACCGTCTCCACTTCCCACCCTC430.15411633991613205No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGTCTCACTTCCCACCCTC330.11827533063331064No Hit
TAAGTAGAGTCTTGGGAAAGGACGAAACACCGTCTCCACTTCCCACCCTC310.11110712877674635No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGGCTCACTGTCTCTCTCT300.10752302784846422No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)