FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_RELA_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_RELA_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59137
Sequences flagged as poor quality0
Sequence length300
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTA5505093.08892909684292No Hit
TAACAATGGTATTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTA4370.7389620711229856No Hit
TAACAATGGGCTTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTA2540.4295111351607285No Hit
TAACAATGGTCTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTAT2250.3804724622486768No Hit
TAACAATGGTCTTGTGGAAGGACGAAACACCGCCCACTGATAGCACCTAT1800.30437796979894144No Hit
TAACAATGGTCTTGTGAAAGGACGAAACACCGCCCACTGATAGCACCTAT1680.28408610514567867No Hit
TAACAATGTCTTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTAT1530.25872127432910025No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCCACTGATAGCACTAT1340.22659248862810086No Hit
TAACAATGGTCTTGTGGAAAGACGAAACACCGCCCACTGATAGCACCTAT1090.1843177706004701No Hit
TAACAATGGTCTTGGGAAAGGACGAAACACCGCCCACTGATAGCACCTAT950.16064392850499687No Hit
TAACAATGGTCTTTGGAAAGGACGAAACACCGCCCACTGATAGCACCTAT840.14204305257283933No Hit
TAACAATGGTTTGTGGAAAGGACGAAACACCGCCCACTGATAGCACCTAT820.1386610751306289No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGCCACTGATAGCACCTAT740.12513316536178704No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)