FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_NFKB2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_NFKB2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14821
Sequences flagged as poor quality0
Sequence length300
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTT925662.45192632076109No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCTCACTGT449930.355576546791713No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGCAATTT640.43181971526887525No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAGGACGAAACACCGGGCTTCCTTG310.20916267458336146No Hit
ACGATCGATAGGTAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG270.18217394237905674No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCTTCCTTG260.17542675932798055No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAGGACGAAACACCGGCTCACTGTC240.1619323932258282No Hit
ACGATCGATAGGTAAGGTCTGTGGAAAGGACGAAACACCGGGCTTCCTTG240.1619323932258282No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAGGACGAAACACCGGGCTTCCTTG210.1416908440725997No Hit
ACGATCGATAGGTAAGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG190.12819647797044734No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCTTG170.11470211186829497No Hit
ACGATCGATAGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG150.10120774576614265No Hit
ACGATCGATAGGTAAGGTCTGTGGAAAGGACGAAACACCGGCTCACTGTC150.10120774576614265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)