FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_NFKB2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_NFKB2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23434
Sequences flagged as poor quality0
Sequence length300
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTA2119990.46257574464454No Hit
CGATCGATACCGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTA5422.312878723222668No Hit
CGATCGATACAGGTATTCTTGTGGAAGGACGAAACACCGCAGCTTCTTAA850.36272083297772467No Hit
CGATCGATACAGGTATTCTGTGGAAAGGACGAAACACCGCAGCTTCTTAA690.29444397029956476No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGTTCTTAA640.27310745071263975No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTAA500.21336519586924982No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTTTAA480.20483058803447982No Hit
CGATCGATACAGGTATTCTTGTGAAAGGACGAAACACCGCAGCTTCTTAA400.17069215669539983No Hit
CGATCGATACAGGTATTCTTGTGGAAAGACGAAACACCGCAGCTTCTTAA390.16642485277801486No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTCTTAA380.16215754886062986No Hit
CGATCGATACAGGTATCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA360.15362294102585985No Hit
CGATCGATACAGGTATTTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA350.14935563710847488No Hit
CGATCGATACAGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA290.12375181360416489No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCGCTTCTTAA290.12375181360416489No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACGCAGCTTCTTAA280.11948450968677989No Hit
CGATCGATAAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA280.11948450968677989No Hit
CGATCGATACAGGTATTCTTTGGAAAGGACGAAACACCGCAGCTTCTTAA260.1109499018520099No Hit
CGATCGATACGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA250.10668259793462491No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)