FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_NFKB1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_NFKB1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33892
Sequences flagged as poor quality0
Sequence length300
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCG3139392.6265785436091No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGGAGAGGATTTCGT1820.5369998819780479No Hit
ATCGATTCCTTGGTTCTTGTGAAAGGACGAAACACCGGAGAGGATTTCGT970.2862032338014871No Hit
ATCGATTCCTTGGTTCTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT860.25374719697863807No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGACGAAACACCGGAGAGGATTTCGT790.2330933553640977No Hit
ATCGATTCCTTGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT660.19473622093709428No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAGAGGATTTCGT640.18883512333293995No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGGAGAGGATTTCGT590.174082379322554No Hit
ATCGATTCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT580.1711318305204768No Hit
ATCGATTCCTTGGTTCTTGGGAAAGGACGAAACACCGGAGAGGATTTCGT560.16523073291632245No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTCGT550.16228018411424525No Hit
ATCGATTCCTTGGTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT460.13572524489555057No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGATTTCGT460.13572524489555057No Hit
ATCGATTCCTTGGTTTTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT450.13277469609347337No Hit
ATCGATTCCTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCGT410.12097250088516465No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGGAGGATTTCGT390.11507140328101027No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTCT380.1121208544789331No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGGAGAGGATTTAG350.10326920807270151No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)