FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_MAP3K5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_MAP3K5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18690
Sequences flagged as poor quality0
Sequence length300
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTA1350572.25789192081326No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTT359319.22418405564473No Hit
TAACAATGGTATTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTA1440.7704654895666132No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTC980.5243445692883896No Hit
TAACAATGGGCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTA630.33707865168539325No Hit
TAACAATGGTCTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT570.30497592295345105No Hit
TAACAATGGTCTTGTGGAAGGACGAAACACCGGTGCTGATTGGGCATTAT500.26752273943285176No Hit
TAACAATGGTATTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTT450.2407704654895666No Hit
TAACAATGGTCTTGTGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT390.2086677367576244No Hit
TAACAATGGTCTTGTGGAAAGACGAAACACCGGTGCTGATTGGGCATTAT340.18191546281433923No Hit
TAACAATGGTCTTTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT290.15516318887105404No Hit
TAACAATGTCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT290.15516318887105404No Hit
TAACAATGGGCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGCATTT290.15516318887105404No Hit
TAACAATGGTCTTGGGAAAGGACGAAACACCGGTGCTGATTGGGCATTAT230.12306046013911183No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGTGCTGATTGGGCATTAT200.1070090957731407No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGGTGCTGATTGGGGCATT200.1070090957731407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)