FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_MAP3K14_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_MAP3K14_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5950
Sequences flagged as poor quality0
Sequence length300
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAG554493.17647058823529No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGCTGTGTAGACAGC230.3865546218487395No Hit
ATCGATTCCTTGGTTCTTGTGAAAGGACGAAACACCGCTGTGTAGACAGC190.31932773109243695No Hit
ATCGATTCCTTGGTTCTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC190.31932773109243695No Hit
ATCGATTCCTTGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC140.2352941176470588No Hit
ATCGATTCCTGGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC110.18487394957983194No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGACGAAACACCGCTGTGTAGACAGC100.16806722689075632No Hit
ATCGATTCCTTGGTTCTTTGGAAAGGACGAAACACCGCTGTGTAGACAGC100.16806722689075632No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGCTGTGTAGACAGC90.15126050420168066No Hit
ATCGATTCCTTGGTTTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC90.15126050420168066No Hit
ATCGATTCTTGGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC70.1176470588235294No Hit
ATCGATTCCTTGGTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC60.10084033613445378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)