FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_MAP3K14_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_MAP3K14_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10781
Sequences flagged as poor quality0
Sequence length300
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA977790.68732028568779No Hit
CGATCATGATCGTATTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA950.881179853445877No Hit
CGATCATGATAGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA800.7420461923754754No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAGCCAAGGAGAA540.5008811798534458No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCAAGGAGAA370.3431963639736574No Hit
CGATCATGATCGTCTTGTGGAAGGACGAAACACCGCAAAGCCAAGGAGAA360.3339207865689639No Hit
CGATCATGATCGTCTTGTGAAAGGACGAAACACCGCAAAGCCAAGGAGAA360.3339207865689639No Hit
CGATCATGATCGTCTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA330.30609405435488357No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACGCAAAGCCAAGGAGAA260.24116501252202951No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAGGAGAA230.21333828030794919No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAGCCAAGGAGAA220.20406270290325573No Hit
CGATCATGATCGTTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA220.20406270290325573No Hit
CGATCATGATCGTCTTGGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA210.19478712549856228No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGGAA200.18551154809386886No Hit
CGATCATGATGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA200.18551154809386886No Hit
CGATCATGATCGTCTTGTGGAAAGACGAAACACCGCAAAGCCAAGGAGAA200.18551154809386886No Hit
CGATCATGATCGTCTTTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA180.16696039328448195No Hit
CGATCATGAGCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA150.13913366107040165No Hit
CGATCATGATCGTCTTGTAGAAAGGACGAAACACCGCAAAGCCAAGGAGA120.1113069288563213No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)