FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_LTA_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_LTA_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4502
Sequences flagged as poor quality0
Sequence length300
%GC56

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATTGAGAC418492.93647267880942No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAGGACGAAACACCGGATTGAGACC210.46645935139937805No Hit
ACGATCGATAGGTAGGTCTTGTGGAAAGGACGAAACACCGGATTGAGACC160.3553976010661928No Hit
ACGATCGATAGGTAAGGTCTGTGGAAAGGACGAAACACCGGATTGAGACC100.2221235006663705No Hit
ACGATCGATGGTAAGGTCTTGTGGAAAGGACGAAACACCGGATTGAGACC90.19991115059973344No Hit
ACGATCGATAGGTAAGGTTTGTGGAAAGGACGAAACACCGGATTGAGACC70.15548645046645934No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAGGACGAAACACCGGATTGAGACC70.15548645046645934No Hit
ACGATCGATAGGTAAGGTCTTTGGAAAGGACGAAACACCGGATTGAGACC60.13327410039982232No Hit
ACGATCGATAGGTAAGGCTTGTGGAAAGGACGAAACACCGGATTGAGACC60.13327410039982232No Hit
ACGATCGATAGGTAAGTCTTGTGGAAAGGACGAAACACCGGATTGAGACC50.11106175033318524No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGATTGAGACC50.11106175033318524No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGACGAAACACCGGATTGAGACC50.11106175033318524No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGTGGCTATG50.11106175033318524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)