FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_LIGHT_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_LIGHT_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11720
Sequences flagged as poor quality0
Sequence length300
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTC1066590.99829351535837No Hit
CGATCGATACCGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTC3142.6791808873720138No Hit
CGATCGATACAGGTATTCTTGTGGAAGGACGAAACACCGGAGCAATTTCG490.4180887372013652No Hit
CGATCGATACAGGTATTCTGTGGAAAGGACGAAACACCGGAGCAATTTCG460.3924914675767918No Hit
CGATCGATACAGGTATTCTTGTGAAAGGACGAAACACCGGAGCAATTTCG230.1962457337883959No Hit
CGATCGATACAGGTATCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG180.15358361774744025No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCATTTCG160.13651877133105803No Hit
CGATCGATACAGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG150.12798634812286688No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAACACCGGAGCAATTTCG150.12798634812286688No Hit
CGATCGATACAGGTATTTTGTGGAAAGGACGAAACACCGGAGCAATTTCG150.12798634812286688No Hit
CGATCGATACGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG140.11945392491467575No Hit
CGATCGATAAAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTC120.10238907849829351No Hit
CGATCGATACAGGTATTCTTTGGAAAGGACGAAACACCGGAGCAATTTCG120.10238907849829351No Hit
CGATCGATAAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG120.10238907849829351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)