FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_Mix6NTs_LIGHT_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_Mix6NTs_LIGHT_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10844
Sequences flagged as poor quality0
Sequence length300
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAA998592.07856879380303No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAACAAGCAAC560.5164146071560309No Hit
GATCGATAACGCATTTCTTGTGGAAGGACGAAACACCGCAAACAAGCAAC450.41497602360752495No Hit
GATCGATAACGCATTTCTTGTGAAAGGACGAAACACCGCAAACAAGCAAC360.33198081888601993No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAGCAAC350.32275912947251933No Hit
GATCGATAACGCATTTCTGTGGAAAGGACGAAACACCGCAAACAAGCAAC340.3135374400590188No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAACAAGCAAC260.23976392475101438No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAC220.2028771670970122No Hit
GATCGATAACGCATTTCTTTGGAAAGGACGAAACACCGCAAACAAGCAAC210.19365547768351163No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGAAC210.19365547768351163No Hit
GATCGATAACGCATTTCTTGTGGAAAGACGAAACACCGCAAACAAGCAAC190.1752120988565105No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACGCAAACAAGCAAC180.16599040944300997No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAAAAGCAAC140.12910365178900773No Hit
GATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG130.11988196237550719No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACTAAACACCGCAAACAAGCAA120.11066027296200663No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAACACCGCAAACAAGCAAC110.1014385835485061No Hit
GATCGATAAAGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAA110.1014385835485061No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)