FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_LTBR_NFKB2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_LTBR_NFKB2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14105
Sequences flagged as poor quality0
Sequence length300
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTT1228287.07550514002126No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCAGACTAAC2271.6093583835519318No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGACCTATCC1931.3683091102445941No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCAAACAAGC920.65225097483162No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAGGACGAAACACCGGGCTTCCTTG650.4608294930875576No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGTGAGGCAGC620.43956043956043955No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCTCACTGT460.32612548741581No Hit
ACGATCGATAGGTAAGGTCTGTGGAAAGGACGAAACACCGGGCTTCCTTG450.31903580290677064No Hit
ACGATCGATAGGTAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG440.3119461183977313No Hit
ACGATCGATAGGTAAGGTCTTGTGAAAGGACGAAACACCGGGCTTCCTTG310.21978021978021978No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGCTTCCTTG300.21269053527118043No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTG300.21269053527118043No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGAGCAATTT280.19851116625310172No Hit
ACGATCGATAGGTAAGGTTTGTGGAAAGGACGAAACACCGGGCTTCCTTG250.1772421127259837No Hit
ACGATCGATAGGTAAGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG250.1772421127259837No Hit
ACGATCGATAGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG250.1772421127259837No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACGGGCTTCCTTG240.17015242821694435No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCTTG220.15597305919886564No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGACGAAACACCGGGCTTCCTTG210.1488833746898263No Hit
ACGATCGATGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG180.12761432116270827No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGCAGCTTCTT160.11343495214462956No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTCCTTG160.11343495214462956No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGGCGAAACACCGGGCTTCCTT150.10634526763559021No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)